The king fell into despair at the situation, but Merlin was delighted because the sex of the one to become king had never mattered. She was entrusted to the king's vassals and was to be raised as the child of a mere knight. It is said in the legend that Arthur was left in Merlin's care as the price for his assistance in ensuring the success for Uther's love of Igrane, saying "I will properly guide this child, one bearing a great destiny, and protect him from the crisis of the royal family." The true reality was that Altria was not a boy, so the king could not make a child that was not male his successor even if she was fated to one day become a king. The king, believing in Merlin's prophecy, yearned for the birth of his appointed successor, but the child born was no the one he desired. Altria was born into this period as the heir to the throne, the daughter of the King of Britain, Uther Pendragon, and the wife of his servant, the Duke of Cornwall, the blue-eyed, Igraine. This time of barbarian invasions and self destructive strife between clans started a long period of war that would later become known as the "Dark Ages. Once the country lost the empire's protection, it could not escape becoming independent, causing it to break into smaller countries soon after. In preparation for the war against the barbarians, the empire deprived her island country of any military forces. It had been believed to be indestructible, but it was only awaiting its destruction at the hands of invading barbarians. In Fate/stay-night, the legendary King Arthur has been reimagined as a young woman rather than a man, and deeply burdened with the responsibility of upholding her ideals as what constitutes a rightful king.Īltria was born into a time of chaos and war that began with the demise of an empire. Saber's true identity is Altria Pendragon, better known as Arthur Pendragon and King Arthur.
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![]() The products can be booked through the SIXT app, which also integrates the services of its renowned mobility partners. With its products SIXT rent, SIXT share, SIXT ride and SIXT+ on the mobility platform ONE the company offers a uniquely integrated premium mobility service across the fields of vehicle and commercial vehicle rental, car sharing, ride hailing and car subscriptions. Sixt SE with its registered office in Pullach near Munich, is a leading international provider of high-quality mobility services. Customers are advised to book early and pay in advance via the SIXT website or app to secure the best rates for their premium rentals. With outstanding customer service, technical innovation, and affordable prices on world-class manufacturers, SIXT USA aims to make travelling exciting, easy, and more flexible than customers ever thought it could be. ![]() In 2020, SIXT USA set the course for further growth by acquiring 10 strategically important airport stations and launching the car subscription service SIXT+. SIXT USA opened its first branch in 2011 and recently expanded its position as the fourth-largest car rental company in the US, the world’s largest car rental market with a market volume of more than USD 32 billion. ![]() ![]() SIXT USA has continued to expand operations with over 1,200 employees serving 100 rental locations in 22 states, including California, Florida, New York, Texas, Colorado, and Hawaii. “Charlotte and Baltimore have so much to offer, from their culinary scenes to the incredible festivals they host, and we’re thrilled to equip visitors with the best vehicles to enhance their experiences while exploring these destinations.” “As travel continues to boom, we are growing SIXT USA’s presence across the East Coast to offer consumers premium rentals at affordable prices, at these major hubs,” said Michael Meissner, President and Chief Operating Officer at SIXT USA. Since the beginning of 2022, SIXT can now be found at 28 of the top 30 airports in the US, providing increased support to traveling customers and the local communities. In addition, SIXT USA invested in the Tampa market by doubling their footprint at Tampa International Airport (TPA). This follows the opening of three new locations across the country including Cincinnati/Northern Kentucky International Airport (CVG), Albuquerque International Sunport Airport (ABQ), and Portland International Airport (PDX) at the end of last year. Expanding to major airport and business hubs like BWI and CLT, two of the top 30 rental car markets in the country, enables SIXT USA to further support its growing corporate customer base and strengthen the east coast network. BWI was recently named the best in the world for passenger satisfaction, serving up to 25 million passengers annually, according to the Airport Council International Airport Service Quality survey. Last week, the premium mobility provider opened its Charlotte Douglas International Airport (CLT) location, the second largest hub for American Airlines, and recently celebrated the opening of its Baltimore/Washington International Thurgood Marshall Airport (BWI) branch in March 2022. FORT LAUDERDALE, Fla.-( BUSINESS WIRE)-SIXT, one of the leading global mobility providers, is continuing its rapid expansion across the US and announces the opening of new branches in Charlotte and Baltimore, offering customers more rental choices on the East Coast. To get this working in a restrictive fullscreen windows, it was sufficient to call the #UseHook directive at the top of my script. Hi there, Its possible that your keyboard has become defective. VA_SetMasterVolume accepts values between 0.0 and 100.0, inclusive. Since there doesn't seem to be a built-in function to change the volume relatively, you'll have to get the current volume first and then in-/ decrease it at will (here: 5). Of course, you can specify any other hotkey. It overwrites both keys to do the same as before, but instead of relying on the OS to catch the keypress, AutoHotkey sets the volume by itself. In principle, this code contains everything you'll need. 03 / Make sure the Startup Type is set to 'Automatic' (Fig. 02 / Scroll through and open 'Human Interface Device Access' (Fig. Hi e1 my keyboard is going crazy, I spilled my drink and hitted the arrow. 01 / If the media keys on the keyboard arent working properly, please use Windows Search tool to look for and open 'Services.msc' (Fig. The functions are pretty straightforward, so I'll just post a little example: Volume_Up:: The dedicated volume and multimedia controls allow you to adjust your audio. The purpose was to be able to control master volume while running restrictive, key press consuming fullscreen applications/games ( Bethesda's games as an infamous example). # choose suitable icon (modeled after the default Ubuntu 12.I stumbled across an AutoHotKey_L library while trying to overwrite my keyboard's Volume_Up and Volume_Down global hotkeys. Everyone thought that the problem is mechnical, BUT If you delete installed drivers in 'Devices and printers' then connect keyboard to another USB ports broblem will dissapear. "sh ~/.volumeHack.sh -c down -i 2% -m Master" Have you ever had a time when you wanted to adjust the volume of the music that you are listening to but youre unable to get it to the specific level you were. Hi everyone, im shure that many people have (had) problems with multimedia scroll on elite versions of keyboards Huntsman and Blackwidow. "sh ~/.volumeHack.sh -c up -i 2% -m Master" I could open it to see if I could fix it but will probably just request a RMA as it's still under warranty. After much frustration and the volume constantly going to zero ( even holding the + volume rocker button didn’t work as the button was working against me constantly resetting to zero and same for the onscreen icon) and freezing my laptop making it virtually unusable. Tried changing usb and blowing the dust.Solution that worked for me was just keep scrolling very fast with smoo. I had this problem on my dell xps 12 laptop so I couldn’t even try the mouse solution as I have a touchpad. Has anyone else had this problem occur yet This is surprising that a premium 230 keyboard would have such a low durability weak point. I had issue with inconsistent volume control. Then edit the file ~/.xbindkeysrc and copy & paste the text below at the bottom of this file. So I've had my K100 since December 2020 and a couple days ago the volume scroll wheel stopped working. all the other keys work fine including the volume mute button. i can scroll up an down but it is still unresponsive on my computer. it was working the first few days i got it, however recently when i was messing around with my lighting i realized my volume scroll wheel is not working at all. Shift, Num(eric) Lock, Scroll Lock, Insert Ctrl, Alt Position cursor. volumeHack.sh and copy and paste the contents below into that file i.e. hi, im having an issue with my volume scroll on the logitech g910 orion spectrum. INTRODUCTION Keyboards allow informational interaction with machines (computers. Using your favourite text editor, create a file in your home folder called. ![]() ![]() Install the xbindkeys package (using Synaptic, or with sudo apt-get install xbindkeys). ![]() With this solution, the default volume step will be reduced to two-percent steps. However I couldn't get it to work - thus my take on the solution is based upon that post. Post #18 in the link gives an interesting workaround which involves using alsamixer increment and decrement capability together with sending notifications to the desktop. Thus, there isn't a simple straightforward configuration change that you can make to reduce the volume step. From this bug-report it appears the volume-step key disappeared in 11.10 and has not (as yet) reappeared. ![]() In fact, any object affected by this “green screen bounce” looks like it is surrounded by a green halo, which is not what you want at all. You get green fuzzy edges around your object, and the result is that your actor looks like they have a green reflection on their skin. Green Screen Color SpillĪ green screen color spill occurs when you have too much light on the green screen, something that might happen if you’re trying to eliminate shadows on a green screen. To avoid motion blur problems, shoot at a higher frame rate (like 120 frames per second) then in post-production interpret the footage at 30 frames per second, and speed up the clip 4 times. Shutter speed can help correct this problem. A motion blur problem occurs when your actor is moving so quickly it’s hard for the camera to pick up the motion against the green screen, and the fastest-moving objects tend to blend in with the screen. Motion Blurįor most corporate videos, this probably won’t be a problem, but if you do have any fast action in your video, you’ll need to watch out for motion blur. Properly lighting the green screen area is essential to the appearance of the final product. However, the easiest way to solve this problem is to prevent it from happening in the first place by using a green screen studio and video production company that is experienced in delivering high-quality green screen videos. Professional video editors can use tools like Adobe After Effects to adjust the lighting. The Chroma key process allows video editors to remove a particular color from the image, but when there are different variations of the color because of uneven lighting, it can be more difficult to remove unwanted colors without also clipping away parts of the subject matter. With this common mistake, lighting may be very uneven, so there are unintentional dark and bright areas on the green screen. Try adding more lighting, reconfiguring your lighting, or moving the subject away from the green screen so they cast less of a shadow in order to eliminate shadows on the green screen. This is why professional lighting is so crucial during green screen filming. The only problem is that if your shadow is too strong, anything you do to try to eliminate it may also eliminate part of the subject that casts the shadow. You don’t want the shadow to appear in your final product, so you will have to eliminate it in editing. Top Green Screen Mistakes Shadows on the Green ScreenĪ shadow on your green screen can create a real problem. Here are some of the top green screen mistakes and what you can do to avoid them. It sounds simple enough, but there are a number of green screen mistakes that can affect the quality of your video. You can rent a green screen production facility for this purpose, or purchase your own green screen and film wherever you like. You film your subject in front of a green screen background, and then using video editing software, the green is stripped (keyed or just removed) out and replaced with whatever background you choose to insert. ![]() A green screen is a simple method of setting your video in a location without actually traveling to that location. Until then, you can avoid using the WQHD resolution in Natural color mode to save yourself some flickering.If you’re making a corporate or educational video, there’s a good chance you’ll want to use a green screen. The moderator confirmed Samsung had already developed a fix, and will seed it soon. Our Samsung Galaxy S22 Ultra review unit is also flickering, suggesting this not an isolated accident. Once these options are enabled, the flicker can happen while playing YouTube videos or unlocking the device. ![]() The issue occurs when the resolution is set at WQHD, and Screen mode is set to Natural. gjznCHTTX2- Golden Reviewer February 22, 2022 (Notice the graphic glitches/artifacts on lock screen clock.) If you have an Exynos #GalaxyS22Ultra pls check if your device is affected by this bug, too. He also assured them a fix is coming soon. Yet some users got their units early and some of those stumbled upon an unpleasant surprise - the screen started flickering in certain scenarios.Īn official forum moderator replied to concerned users on Reddit acknowledging the issue. Samsung launched the Galaxy S22 Ultra on February 9 and promised shipping will begin on February 25. ![]() If a customer sells a vehicle to a Jaguar Land Rover retailer, the retailer, as part of the purchasing process, will check that the customer has cleared all of their accounts and removed the vehicle from their InControl Portal. ![]() In a lengthy statement, the car maker went on to defend its procedure around the sale of connected cars against criticism from techie drivers we've spoken with. "Matt's situation could have been handled a lot better, with him receiving incorrect information throughout the process," it said. This account – which ties into the InControl service offered by JLR – needs the VIN/car data removed from it when a car changes hands.Įl Reg contacted Jaguar Land Rover's press office about the issue. I don't know how you do this but the current process is clearly not sufficient."Īccording to another secondhand Land Rover driver and IT industry pro, who did not wish to be named, the issue is not just around the mobile app but also the online account with JLR. JLR needs a bullet-proof method for this to be automatically disconnected when the vehicle changes hands. Watts told El Reg: "Data is being collected about me and the vehicle's location and simply provided to whomever previously connected the app to the car. ![]() Someone else has access to a significant amount of data about myself and my vehicle and there appears to be nothing that the manufacturer is prepared to do about it. The previous owner of my car has control over it, they can unlock it, they can remotely set the climate control without me knowing about it, even when the car isn't running, they potentially can even look at the sat-nav system, they can also call break down services to the vehicle and all of this without me knowing anything about it. ![]()
We hope that the present study might serve as a theoretical basis for the development or improvement of TaqMan qPCR/RT-qPCR assays for the detection of highly variable nucleic acid templates. The utility of the dual- probe approach was demonstrated on practical examples by using field specimens. On the other hand, the second probe additively contributed to the overall fluorescence signal. ![]() Our results based on a synthetic amplicon suggest that the second probe does not compromise the TaqMan qPCR/RT-qPCR parameters, which repeatedly and reproducibly remained comparable to those of the corresponding single- probe assays, irrespective of the relative probe orientation, whether opposite or tandem, and probe modifications or combinations thereof. In the present study, we evaluate a TaqMan protocol using two identically labelled hydrolysis probes (simple, LNA (locked-nucleic-acid)) and MGB (minor-groove-binder) modified probes and combinations thereof in a single assay. However, how this alteration influences the reaction parameters has not been comprehensively demonstrated. One option for compensating for such deficiency is to integrate a second identically labelled probe in the assay. Specific concerns are related to false negativity due to probe binding failure. Ongoing evolution of viral pathogens is a significant issue in diagnostic virology employing TaqMan qPCR/RT-qPCR. Nagy, Alexander Vitásková, EliÅ¡ka ÄŒernÃková, Lenka KÅ™ivda, Vlastimil JiÅ™incová, Helena Sedlák, Kamil HornÃÄková, Jitka HavlÃÄková, Martina In conclusion, a simple, rapid method, with high specificity and stability, for the detection of the KIT genotype in pigs was established using TaqMan MGB probe real-time quantitative PCR.Įvaluation of TaqMan qPCR System Integrating Two Identically Labelled Hydrolysis Probes in Single Assay Furthermore, use of the MGB probe set resulted in detection of the target mutation at a high resolution and stability standard curves illustrated that the amplification efficiencies of KIT1 (G) and KIT2 (A) were approximately equal (98.8% and 97.2%). The amplification efficiencies for KIT and ESR were approximately equal, indicating ESR was an appropriate control gene. The CT values among dilutions were significantly different (p < 0.001) and the coefficients of variation from each dilution were low (from 0.13% to 0.26%). A series of parallel amplification curves with the same internal distances were obtained using gradually diluted DNA as templates. ![]() Based on the sequence of the resulting amplified fragment, an MGB probe set was designed to detect the ratio of splice mutation to normal using FQ-PCR. Second, to detect the splice mutation ratio of the G:A substitution in intron 17, a 175 bp region, including the target mutation, was amplified from genomic DNA. A real-time fluorescence-based quantitative PCR (FQ-PCR) protocol was developed to accurately detect KIT CNVs. The single-copy gene, estrogen receptor (ESR), was used as an internal control. First, to detect KIT copy number variation (CNV), primers for exon 2 of the KIT gene, along with a TaqMan minor groove binder ( MGB) probe, were designed. The purpose of this study was to establish a simple, rapid method to determine KIT genotype in pigs. The dominant white phenotype in domestic pigs is caused by two mutations in the KIT gene: a 450 kb duplication containing the entire KIT gene together with flanking sequences and one splice mutation with a G:A substitution in intron 17. Li, Xiuxiu Li, Xiaoning Luo, Rongrong Wang, Wenwen Wang, Tao Tang, Hui Detection of KIT Genotype in Pigs by TaqMan MGB Real-Time Quantitative Polymerase Chain Reaction. ![]() In the highly technological world of Stark, dream machine use has proliferated, giving rise to an increasing number of junkies addicted to lucid dreams. (If you need to refresh your memory, developer Red Thread Games has posted a recap video.) Book One opens in The Storytime, a snowy dreamscape where Zoë has been trapped for nine months while she lies unconscious in a Casablanca hospital. Dreamfall itself is more important because, as one character even points out, it was only the first half of the story. ![]() ![]() In general, playing The Longest Journey isn’t necessary to enjoy this first episode of Dreamfall Chapters. This time it was planned as a single, non-episodic game, but with production taking longer than anticipated, the developers reversed course again, announcing that they would split the game into five “books.” So here we are, finally able to see the end of Zoë, Kian, and April’s stories… or, at least, the beginning of the end. In 2012, series creator Ragnar Tørnquist licensed the IP from his former employer and Kickstarted Chapters for just over $1.5 million. For much of the time in between, it seemed we’d never know the fates of Dreamfall’s three protagonists: Zoë Castillo, a listless college dropout whose quest to find her missing ex-boyfriend entangled her in a corporate conspiracy Kian Alvane, an Azadi assassin questioning his faith on a mission to take down Marcurian rebels and of course April Ryan, the world-hopping, balance-restoring, art-student-turned-freedom-fighter who first appeared in 1999’s The Longest Journey.ĭreamfall Chapters was announced in 2007 as an episodic game, then shelved without ever going into production. These episodic write-ups are based on their original releases and do not reflect any backdated changes made since time of writing.Īs a sequel to a 2006 game that ended with major cliffhangers, Dreamfall Chapters has been a long time coming. Note: Various content updates have been applied to Dreamfall Chapters since it began in October 2014, addressing graphical, technical, and gameplay issues. Taking It to Extremes: Mix insane situations - erupting volcanoes, nuclear meltdowns, 30-foot waves - with everyday tech. Tech Enabled: CNET chronicles tech's role in providing new kinds of accessibility. September saw eBay using the iPhone X camera to let people shop by moving their head, and in July, Microsoft announced its Xbox Adaptive Controller. its not as polished or pleasing to the eye. apparently voice access in android 11 works offline. see if you can get it working without internet you might to download additional files or change a setting or two. Tech companies are working to help people with disabilities access their services - Google in August said it's working with GN Hearing to make a low-power hearing aid with streaming support that can connect with future versions of Android. iirc google assistant work offline but with less accuracy to recognise commands and access to services. Google didn't immediately respond to a request for additional comment. To use voice typing, youll need to be connected to the internet, have a working microphone, and have your cursor in a text box. The feature requires Android 5.0 - Lollipop - or later. The Google Voice Interaction API lets an activity interact with the user using speech to get input that: confirms an action (for example, 'are you sure') selects from a list of options Any. Google analyzes sound picked up by device’s microphone in intervals of a few seconds or less. To activate it, open your device's settings app, go to Accessibility and tap Voice Access, and you'll be able to toggle it in on. To turn it off or on, open Google Now or the Google app and touch Menu > Settings > Voice > Hotword detection. It recommends that you activate the "Ok Google" detection from any screen, get the Google Now Launcher and download a language for offline speech recognition. ![]() The Ghiblers is a podcast devoted exclusively to the movies of Studio Ghibli. All true Ghibl Freaks should have at least one or two videotapes to show off. Until then, why not add this VHS box set to your collection? I found it on Ebay fairly easily. Send them a photo of a twenty-dollar bill and scribble the words, "This can all be yours." The next time you purchase the latest Blu-Ray from GKIDS, send them a nice letter asking them to release this title as well. Instead, it became Japan's all-time box office champion, bring fame, fortune and international acclaim to the director and his studio. Had the movie failed at the box office, he would have been ruined. You follow Miyazaki on the long and difficult journey to create what was, then, his most ambitious film project. It goes into the production of their landmark blockbuster movie in exquisite detail, from conception to completion. Let us hope that Studio Ghibli will indeed release this great program on our shores. Simply taking your ball and going home accomplishes nothing. We would gladly pay money for a separate release, or a reissue of Princess Mononoke with the documentary as a bonus feature. We would love to buy this series on DVD or Blu-Ray. It's quite another thing when said programs are unavailable anywhere. It's perfectly understandable to protect your rights when your movies are easily available on store shelves and online retailers. I have never understood the reasoning behind this strategy. More tragedy: recently, Studio Ghibl had all of these Mononoke documentary videos removed from YouTube on copyright grounds. This is why the YouTube videos were never fully completed. He informed me that the woman who was responsible for the English subtitles was stricken by illness, and she was unable to complete her translation project. Just recently, a Ghibli Blog follower sent me an email about this series. These were the videos that I shared on Ghibli Blog in 2008 (parts one, two, and three). Thankfully for fans, an English translation of the Japanese language arrived from the fan underground, and video episodes soon appeared on YouTube for all to enjoy. It's very unfortunate, because this terrific program deserves to be seen by as wide an audience as possible. ![]() Unfortunately, this documentary has never been released outside of Japan. Even if you can no longer play videotapes, it's nice to have these sitting on your shelf, filling out your prized Ghibli fan collection. These sort of deluxe packages were common in the latter days of VHS, and have always remained prized collectors' items. Here are some cool screenshots from the VHS edition, containing the series on three tapes and encased in a stylish box. This television program originally aired on Japanese network NHK, which has had a very long and fruitful relationship with Hayao Miyazaki, going back to his 1978 series Future Boy Conan, which aired on the network. A decade ago (can hardly believe it's been that long), I posted a series of YouTube video episodes from the DVD documentary How Princess Mononoke Was Born. |
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